Protein A/G is a recombinant fusion protein that combines IgG binding domain from Protein A and Protein G. Protein A/G containing four Fc-bound domains from Protein A and two from Protein G, resulting in the final mass of 50,460 dose. Protein A/G bonding is less dependent on pH than Protein A, but instead has the added properties of Protein A and G.
The A/G protein binds all the subgroups of human IgG, making it useful for purifying polyclonal or monoclonal IgG antibodies of undetermined subclasses. In addition, it binds IgA, IgE, IgM and (to a lesser extent) IgD. The A/G protein also binds all subclasses of mouse IgG but does not bind to the mouse IgA, IgM or serum albumin. This allows Protein A/G to be used for the purification and detection of mouse monoclonal IgG antibodies, without interference from IgA, IgM and serum albumin. Rat monoclonal antibodies generally have a stronger affinity for Protein A/G chimeric than Protein A or Protein G. Protein A/G has also been used for purification of ape IgG.
Video Protein A/G
Other antibody binding proteins
In addition to Protein A/G, other immunoglobulin binding protein proteins such as Protein A, Protein G and Protein L are all commonly used to purify, paralyze or detect immunoglobulins. Each of these immunoglobulin binding proteins has different antibody binding profiles in terms of the portion of known antibodies and species and types of antibodies.
Maps Protein A/G
References
Source of the article : Wikipedia
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