Dynabeads are superparamagnetic spherical polymer particles of uniform size and a consistent and undefined surface for the adsorption or coupling of various molecules or bioreactive cells.
Video Dynabeads
Description
Dynabeads were developed after John Ugelstad succeeded in creating uniform polystyrene spherical beads (defined as microbeads) of exactly the same size, at Trondheim University, Norway in 1976, something that was otherwise only achieved by NASA in SkyLab weightless conditions. Dynabeads typically have a diameter of 1 to 5 micrometers. This is in contrast to the magnetic-activated cell randomisation beads, which are approximately 50 nm.
This invention revolutionizes the liquid-phase kinetic segregation of many biological materials. The technology behind the beads, called Dynabeads, was licensed to Dyno Industrier in 1980 and this magnetic separation technology has been used for the isolation and manipulation of biological materials, including cells, nucleic acids, proteins and pathogenic microorganisms. Uniformity in size, shape, and surface area allows for reproducibility and helps minimize chemical agglutination.
Dynabeads are often used for cell isolation . Types of cells that are often attractive to purify may be specific leukocytes, such as CD4 T cells, stem cells, or circulating tumor cells (CTCs). Dynabeads may be covalently connected to antibodies that recognize specific proteins on the surface of the target cell type. Alternatively, Dynabeads may attach to the cell indirectly, either through streptavidin in Dynabead connecting to primary antibodies biotinylated, or secondary antibodies to Dynabead connecting to primary antibodies. Streptavidin linkage to primary antibodies allows Dynabeads to capture cells with lower surface protein expression.
Following a series of mergers and acquisitions, Dynal and Dynabeads are currently owned and manufactured by Invitrogen, part of Thermo Fisher Scientific.
Maps Dynabeads
See also
- Flow cytometry
- Cell sorting is magnetic enabled
References
Further reading
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Ashok Kumar; Igor Yu Galaev; Bo Mattiasson (2007). Cell separation: basics, analytical methods and preparation . Jumper. ISBN: 978-3-540-75262-2.
External links
- Bead separator eBook
Source of the article : Wikipedia
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